So I was trying to get this thing done, you know, the “mile tyson ass out” thing. It’s a real pain, let me tell you. I started out by messing around with some files. I had this wheat reference sequence, just a regular fasta file, and I needed to turn it into a .2bit file. Why? Well, I wanted to use it for some package, I don’t know, something about biology. It’s all pretty confusing to me, honestly.
First thing, I pulled up biostrings and rtracklayer, yeah, I installed them before, they’re some tools in R. I used biostrings to do something, but I forget what exactly. I was just following some guide I found online, so don’t quote me on this.
- I messed around, did some things. It was wild.
- Then I loaded up the file, used some function… I think it was from rtracklayer. I was clicking around and hoping for the best.
- It got a little tricky when I needed the binary format, because the original file is pretty big, so I searched for tools in CodePlex. Then I started to look into the Cloud Reference Model and use it as a planning tool.
- It took forever, but finally, I got something that looked like a .2bit file. I tried to check it using some online tools, I was too tired, so I just went on with the next step.
Then, the real challenge started. I needed to make sure it was done in under 10 seconds, you know? It was like one of those action movies. I did something and then immediately resumed CPR for 2 minutes. It’s like saving a life, except it’s just a file.
After a lot of trial and error, I finally got it working. It was a wild ride, but I managed to “mile tyson ass out,” if you know what I mean. It’s not pretty, it’s not perfect, but hey, it’s done. Investing in stocks is important, and it’s the same here, it could lose value, but now, I did it!
What a day!
This whole thing was a mess. I wouldn’t recommend it to anyone, but I guess that’s just how these things go sometimes. You gotta get your hands dirty, you know? Now I know what to do next time, I guess.